1、水禽泄殖腔棉拭子中禽流感病毒的分离与纯化莫一群，韩涛，过佳朦，刘坚，朱水明，朱星熠，王晓泉*扬州大学兽医学院，扬州，江苏 225009摘要：禽流感病毒(Avian influenza viruses,AIVs)是重要的动物传染病病原，其自然宿主为水禽。除了AIV，水禽常常会共感染其他病毒，如副粘病毒，其中新城疫病毒（Newcastle disease virus，NDV)由于某些毒株对家禽高度致病也具有特别意义。水禽常规AIV监测主要是通过采集大量的泄殖腔棉拭样品，这些样品通过荧光定量PCR（real-time reverse transcription polymerase chain re
2、action，rRT-PCR）检测为阳性，但是鸡胚未分离到病毒。进一步检测发现，这些样品常规RT-PCR检测NDV阳性。假设如果一个样品中同时存在NDV和AIV，NDV可能超过AIV生长产生AIV假阴性。进一步检测发现，这些样品RT-PCR检测NDV阳性。这类样品经过鸡NDV多抗血清处理，再接种鸡胚。几个假阴性样品都发现存在不同亚型的AIV，说明存在混合感染NDV和AIV。在AIV监测中，有必要消除NDV的影响，以获得AIV真正的流行情况。同时获得水禽体内的NDV循环信息，阐明了其流行病学和生态学意义。关键词：禽流感，泄殖腔棉拭样品，混合感染，新城疫，水禽莫一群:年龄，电话，传真，邮箱*通讯作
3、者，tel:+86 514 8797 2247;fax:+86 514 8797 2591;e-mail:springyzyahooIsolation and purification of Avian influenza virus(AIV)from cloacal samples of waterfowlYiqun Mo,Tao Han,Jiameng Guo,Jian Liu,Shuiming Zhu,Xingyi Zhu,Xiaoquan Wang(College of veterinary medicine,Yangzhou University,Yangzhou,Jiangsu 2
4、25009,China)Abstract:Avian influenza viruses(AIVs)are important zoonotic pathogens whose natural reservoir is waterfowl.In addition to AIV,waterfowl are often coinfected with other viruses,such as the paramyxoviruses,of which Newcastle disease virus(NDV)is of particular importance because of the hig
5、hly virulent nature of certain strains of this virus for domestic poultry.In routine surveillance of waterfowl for AIV,a number of cloacal samples were encountered that were positive for AIV by real-time reverse transcription polymerase chain reaction(rRT-PCR),but did not yield AIV by inoculation in
6、 embryonated chicken eggs.On further testing,these samples were also positive for NDV by conventional RT-PCR.It was hypothesized that if both NDV and AIV are present in a sample,the former may overgrow AIV yielding false-negative AIV results.Such samples were treated with chicken anti-NDV polyclonal
7、 antiserum and then inoculated in embryonated chicken eggs.Several samples were found to be positive for different subtypes of AIV,indicating that,in the presence of mixed infection with NDV and AIV,it is imperative to remove the influence of NDV,so a true picture of AIV prevalence emerges.An additi
8、onal benefit is that information on the circulation of NDV in these birds sheds light on（阐明）their epidemiologic and ecologic significance.Key Words:Avian influenza，cloacal samples，mixed infection，Newcastle disease，waterfowl*corresponding author,tel:+86 514 8797 2247;fax:+86 514 8797 2591;e-mail:springyzyahoo
